By Herbert E. Spiegel

ISBN-10: 012010332X

ISBN-13: 9780120103324

Compliment for the sequence: "Its continuity of pertinence, excellence, and authority is still unbroken - a tribute to the skilful enhancing and writing concerned. each knowledgeable laboratory employees should have on hand a duplicate of this volume." --Clinical Chemistry For greater than thirty years, this serial has broadened the technical scope and accelerated the medical base of medical chemistry. those volumes make clear the components of molecular biology, informatics, and the tracking of physiological parameters in serious occasions as they pertain to medical chemistry. each one quantity of Advances in scientific Chemistry comprises an index, and every bankruptcy contains references.

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Yu, S . , Pritchard, M. , Nancarrow, J. , Fragile X genotype characterized by an unstable region of DNA. Science 252, 1179-1 181 (1991). Z I . Zumwalt, R. , Application of molecular biology to forensic pathology. Hum. Purhol. 20, 303-307 (1989). ADVANCES I N CLINICAL CHEMISTRY, VOL. 32 CLINICAL MOLECULAR BIOLOGY: CONCEPTS AND APPLICATIONS Kaiser J. kit" Division of Clinical laboratory Devices Food and Drug Administration Rockville, Maryland 1. Introduction .. .. of Diseases . . . . . . , .

Chromosome structure and terminology. 1 stands for chromosome I , arm q, region 2, band 1, subband 1 . formation arrests the dividing lymphocytes in metaphase. The cells in metaphase are immersed in hypotonic saline to osmotically swell the cells, which are then fixed in methano1:acetic acid. The chromosomal preparations are manipulated to disperse the chromosomes, air dried, and stained. The selected cells are photographed under a microscope, and individual chromosomes from photographs of a single cell are arranged in pairs to form a karyotype.

Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res. 17, 2503-25 I6 (1989). N6. Niesters, H. G. M.. Goessens, W. H. , Meis, J. F. M. , and Quint, W. G. , Rapid polymerase chain reaction-based identification assays for candida species. J . Microbiol. 31, 904-910 (1993). 01. , Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5, 874879 (1989). P I . Poddighe, P. , Moesker, 0..

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Advances in Clinical Chemistry, Vol. 32 by Herbert E. Spiegel

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